Tag
dNLS

Part:BBa_K2549056:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-08)


destroyable nuclear location sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We replaced the spacer with a high-affinity TEV cleavage sequence to make it destroyable. Besides, we insert glycine and serine on both ends of the TEV cleavage sequence to ensure higher flexibility.

More details about TEV protease, please check Part:BBa_K2549013.